# Need help to setup the nf-core-rnaseq with google cloud

**URL:** <https://community.seqera.io/t/need-help-to-setup-the-nf-core-rnaseq-with-google-cloud/2305>\
**Category:** Ask for help\
**Tags:** nextflow, nf-core\
**Created:** [July 30, 2025, 1:49pm UTC](https://community.seqera.io/t/need-help-to-setup-the-nf-core-rnaseq-with-google-cloud/2305 "2025-07-30T13:49:30Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![marvin\_li](https://dub1.discourse-cdn.com/flex013/user_avatar/community.seqera.io/marvin_li/32/1928_2.png) [@marvin\_li](https://community.seqera.io/u/marvin_li)\
**Post date:** [July 30, 2025, 1:49pm UTC](https://community.seqera.io/t/need-help-to-setup-the-nf-core-rnaseq-with-google-cloud/2305/1 "2025-07-30T13:49:30Z")

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I am new to this platform, just set the environment using Google cloud, I am trying to use my data to run the nf-core-rnaseq pipeline. This keeps jumping out when I use it. I don’t know where I should look for it. I used Seqera AI to check the permissions and granted all permissions as instructed.

I don’t have a lot of computer background, and I would greatly appreciate it if you could help me with it.

 ![屏幕截图 2025-07-30 143649](https://europe1.discourse-cdn.com/flex013/uploads/seqera/original/2X/b/b48357bbedbd9b35e9253601061e2c8ea1f67749.png)

 ![屏幕截图 2025-07-30 144454](https://europe1.discourse-cdn.com/flex013/uploads/seqera/original/2X/7/79eefe1aa77b88066ea690ded9ffd6276342b3a6.png)

 ![屏幕截图 2025-07-30 144439](https://europe1.discourse-cdn.com/flex013/uploads/seqera/original/2X/b/b90a1d460d9c4c43a66b3168300c9275c372036b.png)

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<div class="post-metadata">

**Author:** ![marvin\_li](https://dub1.discourse-cdn.com/flex013/user_avatar/community.seqera.io/marvin_li/32/1928_2.png) [@marvin\_li](https://community.seqera.io/u/marvin_li)\
**Post date:** [July 30, 2025, 4:06pm UTC](https://community.seqera.io/t/need-help-to-setup-the-nf-core-rnaseq-with-google-cloud/2305/2 "2025-07-30T16:06:21Z")

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I set more admir role on the account, and it seems the problem is solved.

However, if I only use the genome EB2 as a reference, how do I avoid uploading the FASTA genome file and the GTF file? If not, where can I find a GTF file for B. subtilis 168?

here is the log file, many thanks if you can help me with it.

[nf-58hp3WzRnLcwXd.log](https://community.seqera.io/uploads/short-url/l5KAvFJqeZ8dGnCb2PR6tK9ydAN.log) (19.6 KB)