# Nf-core training **URL:** \ **Category:** Training\ **Created:** [February 12, 2026, 1:30pm UTC](https://community.seqera.io/t/nf-core-training/2553 "2026-02-12T13:30:14Z")\ **Posts on this page:** 12\ **Page:** 1
**Author:** ![emma](https://dub1.discourse-cdn.com/flex013/user_avatar/community.seqera.io/emma/32/2269_2.png) [@emma](https://community.seqera.io/u/emma)\ **Post date:** [February 12, 2026, 1:30pm UTC](https://community.seqera.io/t/nf-core-training/2553/1 "2026-02-12T13:30:14Z")
Hello! I’m currently following the training material of the “Hello nf-core” training. In part 2 I’m trying to run the pipeline with the test profile. However for some reason I keep getting an error, and I can’t figure out why. ```auto training/hello-nf-core → nextflow run core-hello --outdir core-hello-results -profile test,docker --validate_params false Nextflow 25.10.4 is available - Please consider updating your version to it N E X T F L O W ~ version 25.10.2 Launching core-hello/main.nf [ridiculous_boyd] DSL2 - revision: b9e9b3b8de WARN: Unrecognized config option ‘validation.defaultIgnoreParams’ WARN: Unrecognized config option ‘validation.monochromeLogs’ Input/output options outdir : core-hello-results Generic options validate_params : false trace_report_suffix: 2026-02-12_13-19-37 Core Nextflow options runName : ridiculous_boyd containerEngine : docker launchDir : /workspaces/training/hello-nf-core workDir : /workspaces/training/hello-nf-core/work projectDir : /workspaces/training/hello-nf-core/core-hello userName : root profile : test,docker configFiles : /workspaces/training/hello-nf-core/core-hello/nextflow.config !! Only displaying parameters that differ from the pipeline defaults !! ERROR ~ Validation of pipeline parameters failed! – Check ‘.nextflow.log’ file for details The following invalid input values have been detected: Missing required parameter(s): input –validate_params (false): Value is [string] but should be [boolean] ``` Can somebody please help me fix this? Thanks! ---
**Author:** ![GeraldineVdA](https://dub1.discourse-cdn.com/flex013/user_avatar/community.seqera.io/geraldinevda/32/178_2.png) [@GeraldineVdA](https://community.seqera.io/u/GeraldineVdA)\ **Post date:** [February 12, 2026, 1:37pm UTC](https://community.seqera.io/t/nf-core-training/2553/2 "2026-02-12T13:37:17Z")
Hi @emma , can you specify exactly which exercise you are on? ---
**Author:** ![ewels](https://dub1.discourse-cdn.com/flex013/user_avatar/community.seqera.io/ewels/32/1_2.png) [@ewels](https://community.seqera.io/u/ewels)\ **Post date:** [February 12, 2026, 1:41pm UTC](https://community.seqera.io/t/nf-core-training/2553/3 "2026-02-12T13:41:42Z")
Please confirm that you have done the environment setup steps to do with the Nextflow version: [Getting started - training.nextflow.io](https://training.nextflow.io/latest/hello_nf-core/00_orientation/#version-requirements) Specifically, this: ```auto export NXF_SYNTAX_PARSER=v1 ``` I think that this should solve the error. See the link above for more details. These problems are already fixed in Nextflow dev, so should be solved in the next stable release 🙂 Phil ---
**Author:** ![emma](https://dub1.discourse-cdn.com/flex013/user_avatar/community.seqera.io/emma/32/2269_2.png) [@emma](https://community.seqera.io/u/emma)\ **Post date:** [February 12, 2026, 1:47pm UTC](https://community.seqera.io/t/nf-core-training/2553/4 "2026-02-12T13:47:30Z")
Hi! This works, thank you!! ---
**Author:** ![Rachel](https://dub1.discourse-cdn.com/flex013/user_avatar/community.seqera.io/rachel/32/2268_2.png) [@Rachel](https://community.seqera.io/u/Rachel)\ **Post date:** [February 12, 2026, 5:33pm UTC](https://community.seqera.io/t/nf-core-training/2553/5 "2026-02-12T17:33:29Z")
Hi, when im trying to run the demo pipeline on local computer, it shows errors like this: Is this because of failing to link with URL ? ![image](https://europe1.discourse-cdn.com/flex013/uploads/seqera/original/2X/5/58cd3b6584389031a87ee0deb793996b9bb57894.png) ---
**Author:** ![GeraldineVdA](https://dub1.discourse-cdn.com/flex013/user_avatar/community.seqera.io/geraldinevda/32/178_2.png) [@GeraldineVdA](https://community.seqera.io/u/GeraldineVdA)\ **Post date:** [February 12, 2026, 5:41pm UTC](https://community.seqera.io/t/nf-core-training/2553/6 "2026-02-12T17:41:09Z")
Hi @Rachel , can you clarify if you are trying to run this without an active internet connection? ---
**Author:** ![Rachel](https://dub1.discourse-cdn.com/flex013/user_avatar/community.seqera.io/rachel/32/2268_2.png) [@Rachel](https://community.seqera.io/u/Rachel)\ **Post date:** [February 12, 2026, 5:46pm UTC](https://community.seqera.io/t/nf-core-training/2553/7 "2026-02-12T17:46:56Z")
i have an active internet connection (and also VPN on) ---
**Author:** ![GeraldineVdA](https://dub1.discourse-cdn.com/flex013/user_avatar/community.seqera.io/geraldinevda/32/178_2.png) [@GeraldineVdA](https://community.seqera.io/u/GeraldineVdA)\ **Post date:** [February 12, 2026, 6:00pm UTC](https://community.seqera.io/t/nf-core-training/2553/8 "2026-02-12T18:00:36Z")
Ok, then I’m not sure why this isn’t working for you. Can you confirm that you’ve run `export NXF_SYNTAX_PARSER=v1` and your version of Nextflow is 25.10.2 or later? ---
**Author:** ![Rachel](https://dub1.discourse-cdn.com/flex013/user_avatar/community.seqera.io/rachel/32/2268_2.png) [@Rachel](https://community.seqera.io/u/Rachel)\ **Post date:** [February 12, 2026, 6:13pm UTC](https://community.seqera.io/t/nf-core-training/2553/9 "2026-02-12T18:13:55Z")
Yes, ive set up the environment. After i read the error ERROR ~ Validation of pipeline parameters failed! – Check ‘.nextflow.log’ file for details The following invalid input values have been detected: \* --input (/home/rechal2026/.nextflow/assets/nf-core/demo/assets/samplesheet.csv): Validation of file failed: → Entry 1: Error for field ‘fastq\_2’ (/path/to/fastq/files/AEG588A1\_S1\_L002\_R2\_001.fastq.gz): the file or directory ‘/path/to/fastq/files/AEG588A1\_S1\_L002\_R2\_001.fastq.gz’ does not exist (FastQ file for reads 2 cannot contain spaces and must have extension ‘.fq.gz’ or ‘.fastq.gz’) → Entry 1: Error for field ‘fastq\_1’ (/path/to/fastq/files/AEG588A1\_S1\_L002\_R1\_001.fastq.gz): the file or directory ‘/path/to/fastq/files/AEG588A1\_S1\_L002\_R1\_001.fastq.gz’ does not exist (FastQ file for reads 1 must be provided, cannot contain spaces and must have extension ‘.fq.gz’ or ‘.fastq.gz’) → Entry 2: Error for field ‘fastq\_1’ (/path/to/fastq/files/AEG588A4\_S4\_L003\_R1\_001.fastq.gz): the file or directory ‘/path/to/fastq/files/AEG588A4\_S4\_L003\_R1\_001.fastq.gz’ does not exist (FastQ file for reads 1 must be provided, cannot contain spaces and must have extension ‘.fq.gz’ or ‘.fastq.gz’) i realize that maybe the path “assets/samplesheet.csv” is not real. But then why it can work on CI, but cannot work on local?🤔 ---
**Author:** ![GeraldineVdA](https://dub1.discourse-cdn.com/flex013/user_avatar/community.seqera.io/geraldinevda/32/178_2.png) [@GeraldineVdA](https://community.seqera.io/u/GeraldineVdA)\ **Post date:** [February 12, 2026, 6:23pm UTC](https://community.seqera.io/t/nf-core-training/2553/10 "2026-02-12T18:23:06Z")
Did you change the input to the pipeline to use that? As noted in the training lesson [here](https://training.nextflow.io/latest/hello_nf-core/01_run_demo/#33-inputs-and-validation), “An example samplesheet is also provided under the `assets` directory, although the paths in this one are not real.” That file is just given as an example of how the samplesheet should be formatted. Have you tried using `–input [https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet\_test\_illumina\_amplicon.csv`](https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv%60) directly in your command? ---
**Author:** ![Rachel](https://dub1.discourse-cdn.com/flex013/user_avatar/community.seqera.io/rachel/32/2268_2.png) [@Rachel](https://community.seqera.io/u/Rachel)\ **Post date:** [February 12, 2026, 7:21pm UTC](https://community.seqera.io/t/nf-core-training/2553/11 "2026-02-12T19:21:41Z")
Yes. But when i open this link [https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet\_test\_illumina\_amplicon.csv`](https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv%60), it shows 404 not found. when i run “nextflow run check\_url.nf” it shows: Launching `check_url.nf` [furious\_kirch] DSL2 - revision: 8ba99a01c0 SUCCESS: samplesheet\_test\_illumina\_amplicon.csv :~/pipelines/nf-core/demo$ nextflow run nf-core/demo –input [https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet\_test\_illumina\_amplicon.csv](https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv) –outdir demo-results maybe the link of the fastq files are fake? ---
**Author:** ![system](https://dub1.discourse-cdn.com/flex013/user_avatar/community.seqera.io/system/32/2402_2.png) [@system](https://community.seqera.io/u/system)\ **Post date:** [February 19, 2026, 7:22pm UTC](https://community.seqera.io/t/nf-core-training/2553/12 "2026-02-19T19:22:07Z")
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