# Nfcore/rnaseq pipeline never finishes

**URL:** https://community.seqera.io/t/nfcore-rnaseq-pipeline-never-finishes/1946
**Category:** Ask for help
**Tags:** nf-core, platform, aws
**Created:** [March 11, 2025, 6:44pm UTC](https://community.seqera.io/t/nfcore-rnaseq-pipeline-never-finishes/1946 "2025-03-11T18:44:36Z")
**Posts on this page:** 3
**Page:** 1

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### Author: ![JihedC](https://dub1.discourse-cdn.com/flex013/user_avatar/community.seqera.io/jihedc/32/1644_2.png) [@JihedC](https://community.seqera.io/u/JihedC)
#### Post date: [March 11, 2025, 6:44pm UTC](https://community.seqera.io/t/nfcore-rnaseq-pipeline-never-finishes/1946/1 "2025-03-11T18:44:36Z")

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Hi everyone, I am experiencing some difficulties with the nf-core/rnaseq pipeline. It is the first time I run it using an aws environment.  
I followed the setup in the documentation and it worked well for the nf-core/fetchngs pipeline. So I am pretty sure that my environment is correct.

Since friday I have been trying to run the rna-seq pipeline with my samples (there are many) and it keeps on failing after around 8hours, because of an EC2 crash.

When I resume the pipeline it continues and crashes again after several hours. I did this 5 times in total until I got an new error message this evening.

Unable to create Batch Job definition for container image: [public.cr.seqera.io/platform/nf-launcher:j17-24.10.4](http://public.cr.seqera.io/platform/nf-launcher:j17-24.10.4) - Reason: The security token included in the request is invalid. (Service: AWSBatch; Status Code: 403; Error Code: UnrecognizedClientException; Request ID: 20f4b8fc-dd9b-45f0-8241-ee426e2fa843; Proxy: null)

The pipeline would not start.

I am using the seqera dashboard to run the pipeline. Any help or hint would be much apprecitated!

Thanks!

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<div class="post-metadata">

### Author: ![JihedC](https://dub1.discourse-cdn.com/flex013/user_avatar/community.seqera.io/jihedc/32/1644_2.png) [@JihedC](https://community.seqera.io/u/JihedC)
#### Post date: [March 13, 2025, 12:08pm UTC](https://community.seqera.io/t/nfcore-rnaseq-pipeline-never-finishes/1946/2 "2025-03-13T12:08:38Z")

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I fixed it for now by generating a new compute environment.

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### Author: ![system](https://dub1.discourse-cdn.com/flex013/user_avatar/community.seqera.io/system/32/2402_2.png) [@system](https://community.seqera.io/u/system)
#### Post date: [March 20, 2025, 12:09pm UTC](https://community.seqera.io/t/nfcore-rnaseq-pipeline-never-finishes/1946/3 "2025-03-20T12:09:32Z")

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