I am trying to run GitHub - nf-core/sarek: Analysis pipeline to detect germline or somatic variants (pre-processing, variant calling and annotation) from WGS / targeted sequencing over http://genomedata.org/rnaseq-tutorial/HBR_UHR_ERCC_ds_5pc.tar which is essentially 3 reps for condition with paired reads. I guess i can use the patient column as the condition and sample are the reps but i am not sure what to do about the lane column which seems to be required by the sarek?
Faking the column lane information? · Issue #1942 · nf-core/sarek · GitHub seems to have worked, so i have tried to Make `lane` Column Optional in Samplesheet by animesh · Pull Request #1943 · nf-core/sarek · GitHub 
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